Molecular ecology of algae in formation the target ecosystems of the Gurovo quarry 
1Maltsev Y.I., 1Maltseva S.Y., 2Andreeva S.A.
1Bohdan Khmelnytskyi Melitopol State Pedagogical University, Melitopol, Ukraine
2Institute of Plant Physiology RAS, Moscow, Russia
e-mail: ye.maltsev@gmail.com

Вивчення альгоугруповань Гуровського кар’єру методами молекулярної екології 
1Мальцев Є.І., 2Мальцева С.Ю., 2Андреєва С.О.
1Мелітопольський державний педагогічний університет ім. Б. Хмельницького, Мелітополь, Україна
2Інститут фізіології рослин ім. К.А. Тімірязєва, Москва, Росія
e-mail: ye.maltsev@gmail.com
Annotation. Studies conducted on dumps of various origins showed that algae are often found among pioneer organisms on dump substrates. This event was the impetus for a purposeful study of algal communities of dumps and ascertaining the peculiarities of their seral development. Two-year research of the soil algae diversity of the Gurovo quarry using micrographs and DNA barcodes was established annual dynamics of species composition of algae. 

On substrates of various origins, the role of algae is most pronounced in forming pioneer groupings of living organisms. In 2018, the species diversity of algae outside the water habitats of the Gurovo quarry (Tula region) has increased from 39 to 45 species (in comparison with 2016). An increase in the number of diatom algae species was noted. Representatives of the Phylum Xanthophyta and Phylum Eustigmatophyta were marked for the first time for the soils of the quarry. The data acquisition of the abundance and biomass of algae attest to their active participation in soil-forming processes and increase in the biological activity of the substrates in the quarry. The greatest number of the algal cells were found in ecosystems of the Gurovo quarry belonged to cyanoprokaryotes, and then was a complex group of green, yellow-green and eustigmatophycean algae. The largest number of algae was recorded in the territories that enclosed the quarry (162.1±37.5 thousand per 1 g of substrate), and inside the quarry - on the old dump (69.5±3.5). The smallest number of algae in spring was noted in samples from the western wall of the quarry (6.4±0.8). The largest biomass of algae was recorded in the territories that enclosed the quarry (0.052±0.005 mg per 1 g of substrate), and inside the quarry - on the old dump (0.029±0.005). The smallest algal biomass was noted in samples from the western wall of the quarry (0.0003±0.0001).